Autopure protein purification system

Main application:

Protein purification system is mainly used in biopharmaceutical fields such as monoclonal antibodies, recombinant proteins, vaccines, biochemical drugs, antibiotics, natural products and polysaccharides. It is used for the separation and purification of downstream processes of biological products

Product features and advantages:

  • Low pulsation and high precision double plunger infusion pump ensures excellent separation reproducibility

  • All parts of the whole system in contact with liquid are made of biocompatible materials which meets the requirements of FDA, USP VI and other relevant regulations

  • DAD full spectrum direct reading detector to avoid the instability and high failure rate caused by the traditional mechanical switching detector

  • The fixed wavelength detector lamp is long-life LED lamp, with a service life of more than 8000 hours, which reducing the maintenance cost

  • Patented UV detector flow cell, low dead volume, low peak expansion, and improved chromatographic separation

  • High sensitivity on-line pH, conductivity and UV detector, low dead volume and low peak expansion, which improves the chromatographic separation

  • Automatic components such as buffer selector valve, automatic sample injection valve and column position valve are concentrated to improves chromatographic resolution

  • Pre-column, post-column, differential pressure alarm and bubble sensor detection, which greatly protects the safety of the system and chromatographic column

Unique CDsystem chromatography workstation software

Unique CDsystem is a powerful, advanced and stable chromatographic data management system

  • CDsystem is integrates the functions of instrument control, method management, evaluation, standard curve, report editing, user authority management, audit trial, digital signature, network database etc.

  • CDsystem meets the requirements of FDA 21 CFR, Part 11 / GLP / GMP, and meets the requirements of the pharmaceutical industry for data traceability and safety

Configuration list of Autopure series protein purification system:

AutoPure series:

ModelArticle numberDescription
Unique AutoPure25-L104-004-002-2501、 Infusion pump:
1.1 Binary gradient bio inert infusion pump
*Flow rate:
Range: 0.001-25.000 ml/min
Velocity accuracy: 1.2%
Flow rate precision: 0.5%
*Pressure: 0-27.5 Mpa
*Dynamic mixer: 2ml, panel mounted, easy to replace
2、 Automatic Iinjection valve
2.1: automatic sample injection valve, which can be controlled by software, and supports: load / inject / waste
2.2: 1ml sample loop
3、 280nm fixed wavelength detector
3.1: 280nm imported led detector, service life: 8000hr
3.2: Noise: ± 2x10-5Abs
3.3: 2 mm flow cell
4: Conductivity detector:
4.1: range: 0.000-999.99 mS / cm
4.2: accuracy: ±0.02Ms/cm or  ±2%
5: Protein purification chromatography workstation (including brand computer, software and communication module, chromatography workstation software)
Unique AutoPure25-M40204-004-002-2521、 Infusion pump:
1.1 binary gradient bio inert infusion pump
*Flow rate:
Range: 0.001-25.000ml/min
Velocity accuracy: 1.2%
Flow rate precision: 0.5%
*Pressure: 0-27.5 Mpa
*Dynamic mixer: 2ml, panel mounted, easy to replace
2、 Automatic injection valve
2.1: automatic sample injection valve, which can be controlled by software, and supports: load / inject / waste
2.2: 1ml sample loop
3、 DAD detector
3.1: 200-400nm imported dad detector
3.2:Noise: ± 1x10-4Abs
3.3: 2mm flow cell
4: Conductivity detector:
4.1: Range: 0.001-999.99 mS / cm
4.2: Accuracy: ±0.02Ms/cm or  ±2%
5: Protein purification chromatography workstation (including brand computer, software and communication module, chromatography workstation software)

AutoPure100 series:

ModelArticle numberDescription
Unique AutoPure100-L104-004-002-1001、 Infusion pump:
1.1 Binary gradient bio inert infusion pump
*Flow rate:
Range: 0.001-100.000 ml/min
Velocity accuracy: 1.2%
Flow rate precision: 0.5%
*Pressure: 0-10 Mpa
*Dynamic mixer: 2ml, panel mounted, easy to replace
2、 Automatic injection valve
2.1: Automatic sample injection valve, which can be controlled by software, and supports load / inject / waste
2.2: 1ml sample loop
3、 280nm fixed wavelength detector
3.1: 280nm imported led detector, service life: 800hr
3.2: noise: ± 2x10-5Abs
3.3: 2mm flow cell
4: Conductivity detector:
4.1: Range: 0.000-999.99 mS / cm
4.2: Accuracy: ±0.02Ms/cm or  ±2%
5: Protein purification chromatography workstation (including brand computer, software and communication module, chromatography workstation software)
Unique AutoPure100-M40204-004-002-1021、 Infusion pump:
1.1 Binary gradient bio inert infusion pump
*Flow rate:
Range: 0.001-100.000ml/min
Velocity accuracy: 1.2%
Flow rate precision: 0.5%
*Pressure: 0-10mpa
*Dynamic mixer: 2ml, panel mounted, easy to replace
2、 Automatic injection valve
2.1: Automatic sample injection valve, which can be controlled by software, and supports load / inject / waste
2.2: 1ml sample loop
3、 DAD detector
3.1: 200-400nm imported DAD detector
3.2: Noise: ± 1x10-4Abs
3.3: 2 mm flow cell
4: Conductivity detector:
4.1: Range: 0.001-999.99 mS / cm
4.2: Accuracy: ±0.02 mS/cm or  ±2%
5: Protein purification chromatography workstation (including brand computer, software and communication module, chromatography workstation software)

AutoPure150 series:

ModelArticle numberDescription
Unique AutoPure150-L104-004-002-1501、 Infusion pump:
1.1 Binary gradient bio inert infusion pump
*Flow rate:
Range: 0.001-150.000ml/min
Velocity accuracy: 1.2%
Flow rate precision: 0.5%
*Pressure: 0-5 Mpa
*Dynamic mixer: 2ml, panel mounted, easy to replace
2、 Automatic sampling valve
2.1: Automatic sample injection valve, which can be controlled by software, and supports load / inject / waste
2.2: 1ml sample 
3、 280nm fixed wavelength detector
3.1: 280nm imported led detector, service life: 8000hr
3.2: Noise: ± 2x10-5Abs
3.3: 2mm flow cell
4: Conductivity detector:
4.1: Range: 0.000-999.99 mS / cm
4.2: Accuracy: ±0.02 mS/cm or  ±2%
5: Protein purification chromatography workstation (including brand computer, software and communication module, chromatography workstation software)
Unique AutoPure150-M40204-004-002-1521、 Infusion pump:
1.1 Binary gradient bio inert infusion pump
*Flow rate:
Range: 0.001-150.000 ml/min
Velocity accuracy: 1.2%
Flow rate precision: 0.5%
*Pressure: 0-5 Mpa
*Dynamic mixer: 2ml, panel mounted, easy to replace
2、 Automatic sampling valve
2.1: Automatic sample injection valve, which can be controlled by software, and supports load / inject / waste
2.2: 1ml sample loop
3、 DAD detector
3.1: 200-400nm imported dad detector
3.2: Noise: ± 1x10-4 Abs
3.3: 2mm flow cell
4: Conductivity detector:
4.1: range: 0.001-999.99 mS / cm
4.2: accuracy: ±0.02 mS/cm or  ±2%
5: Protein purification chromatography workstation (including brand computer, software and communication module, chromatography workstation software)

AutoPure300 series:

ModelArticle numberDescription
Unique AutoPure300-L104-004-002-3001、 Infusion pump:
1.1 Binary gradient bio inert infusion pump
*Flow rate:
Range: 0.001-300.000 ml/min
Velocity accuracy: 2%
Flow rate precision: 0.5%
*Pressure: 0-5 Mpa
*Dynamic mixer: 5ml, panel mounted, easy to replace
2、 Automatic injection valve
2.1: Automatic sample injection valve, which can be controlled by software, and supports load / inject / waste
2.2: 1ml sample loop
3、 280nm fixed wavelength detector
3.1: 280nm imported led detector, service life: 8000hr
3.2: Noise: ± 2x10-5Abs
3.3: 2 mm flow cell
4: Conductivity detector:
4.1: Range: 0.000-999.99 mS / cm
4.2: Accuracy: ±0.02 mS/cm or  ±2%
5: Protein purification chromatography workstation (including brand computer, software and communication module, chromatography workstation software)
Unique AutoPure300-M40204-004-002-3021、 Infusion pump:
1.1 binary gradient bio inert infusion pump
*Flow rate:
Range: 0.001-300.000ml/min
Velocity accuracy: 2%
Flow rate precision: 0.5%
*Pressure: 0-5 Mpa
*Dynamic mixer: 5ml, panel mounted, easy to replace
2、 Automatic injection valve
2.1: automatic sample injection valve, which can be controlled by software, and supports load / inject / waste
2.2: 1ml sample loop
3、 DAD detector
3.1: 200-400nm imported DAD detector
3.2: Noise: ± 1x10-4Abs
3.3: 2 mm flow cell
4: Conductivity detector:
4.1: range: 0.001-999.99 mS / cm
4.2: accuracy: ±0.02 mS/cm or  ±2%
5: Protein purification chromatography workstation (including brand computer, software and communication module, chromatography workstation software)

Optional configuration:

ModelArticle numberDescription
AirS01-H03-001-003-002Bubble sensor for monitoring sample loading
CPV02RX03-001-001-002Single column position valve, supporting downward / upward / bypass
MSV0403-001-002-003Mobile phase selection module, 4 channels
MSV1003-001-002-002Mobile phase selection module, 10 channels
DAD60403-002-001-003Imported four channel DAD detector
Wavelength range: 200-600nm
Noise: ± 0.04 mAu
Drift: 1x10-3 Au / HR
DAD80403-002-001-004Imported four channel DAD detector
Wavelength range: 200-800nm
Noise: ± 0.04mAu
Drift: 1x10-3 Au / HR
pH detector03-004-001-001pH detector
0-14 (best for ph2-12), temperature compensation
Dead volume of circulation tank: 76μl
OV0203-003-001-001Outlet valve (large volume collection valve), 1 collection channel and 1 waste liquid
OV1003-003-001-001Outlet valve (large volume collection valve), 9 collection channels and 1 waste liquid
Frac-0203-003-001-003Fraction collector
Support 2 collection racks
1. 3x7, 30mm, 21 tubes, 2
2. 5x12, 16mm, 60 tubes, 2
3. 6x15, 13mm, 90 pipe, 2
4. 96 hole plate, 4
BACK