Unique AutoOligo is a flexible, intuitive, automatic oligonucleotide synthesizer for the rapid synthesis of 1μ mol-12mmol nucleic acid samples. It is suitable for clinical research, pharmaceutical development and molecular diagnostic probe synthesis.
Unique AutoOligo has three models:
Unique AutoOligo25, synthetic amount 1 ~ 100 μmol；
Unique AutoOligo100, synthetic amount 50μmol ~ 9mmol；
Unique AutoOligo150, synthetic amount 150μmol ~ 12mmol；
Unique AutoOligo600, synthetic amount 1mmol ~ 50mmol；
● Unique AutoOligo adopts a high-precision metering pump drive system, which is compatible with reagents for nucleic acid synthesis. It adopts flow-through solid-phase column synthesis technology, which can significantly reduce reagent consumption and ensure accurate control of reaction speed and contact time. At the same time, it has higher synthesis efficiency and is convenient for linear amplification.
● Unique AutoOligo can use 14 monomers at the same time, continuously and automatically on 1 or 3 or 8 columns.
● Unique AutoOligo system workstation has a powerful data management system to provide you with a complete and reliable nucleic acid synthesis method development platform.
Unique AutoOligo features:
● High precision metering pump, Flow path is resistant to organic reagents;
● Multiple column positions are optional;
● High degree of automation ;
● Monomer solution recycling;
● Sequential synthesis;
● High capacity: One synthesis cycle can be completed in as little as 20 minutes;
● Online monitoring of UV, conductivity signal and pressure;
● The software interface is friendly and the method can be edited flexibly;
● Comply with relevant regulations of FDA and USP;
instrument performance parameters:
| Chemical solvent compatibility | Compatible with common reagents for DNA and RNA synthesis, acetonitrile, coupling reagent, cap removing agent, oxidation reagent, cap capping agent, sulfide reagent, etc. (not compatible with TETRAhydrofuran) |
| synthetic scale | 1 µmol to 12 mmol |
| synthesis efficiency | >99% for DNA & >98% for RNA |
| cycle time | 4.5 to 25min (Standard DNA) |
| reagent stabilization time in the instrument | > 2 weeks |
Instrument configuration:
| Dimensions (W & H & D) | 400 × 550 × 480 mm |
| Weight | 60 kg |
| Synthetic columns | 1, 3, maximum 8 |
| Number of monomer inlets | standard 8, maximum 14 |
| AutoOligo25、AutoOligo100、AutoOligo150 | 2 × 25 mL/min、2 ×100 mL/min、2 ×150 mL/min |
| Detector | Conductivity, UV detector, UV detector wavelength range: 200-600nm, 4 channels |
| System protection | Argon or nitrogen |
| Air inlet pressure | 0.3~0.35bar |
| Valve | 7 low dead volume valves |
| Control system | Unique AutoOligo System |
| Warranty | 1 year |